Evaluation of bactericidal activity (ability to cause the death of the microorganism) of the substance QP42 in vitro was determined in the automated system Bactec MGIT 960. The precipitate the bacterial cells from the tubes, where growth has been identified in the presence of the substance QP42, washed and inoculated into a test tube with fresh nutrient medium without drug for 42 days. The lack of growth in fresh nutrient medium shows the bactericidal action of the drug. The drug, which has bactericidal properties, is considered more promising, because leads to the elimination of the causative agent. The control compound is rifampicin. The results are shown in Table 1.
| Table. 1. Test results on the bactericidal activity of the drug candidate QP42 | ||
|---|---|---|
| Drug | Dose (mg/ml) | The presence of culture growth, incubation 42 days |
| QP42 | 3,70 | yes |
| QP42 | 11,11 | no |
| QP42 | 33,33 | no |
| QP42 | 100 | no |
| Rifampicin | 0,41 | yes |
| Rifampicin | 1,23 | no |
| Rifampicin | 3,70 | no |
| Rifampicin | 11,11 | no |
| Rifampicin | 33,33 | no |
| Rifampicin | 100 | no |
In conclusion, the studies of bactericidal activity of the drug QP42 on this cultivation in the automated detection system Bactec MGIT 960 showed that it possesses bactericidal activity against M.tuberculosis H37Rv strain at concentrations of 11.11 mg / ml.
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